Oct4 (POU5F1) Mouse Monoclonal Antibody [Clone ID: 5F7]

CAT#: AM06559SU-N

Oct4 (POU5F1) mouse monoclonal antibody, clone 5F7, Ascites

Product Images

Western blot analysis using Oct4 antibody Cat.-No AM06559SU-N against NTERA-2 cell lysate (1).

Specifications

Product Data

• Clone Name: 5F7
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthesized peptide of Human Oct4.
• Specificity: Recognizes OCT3/4
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 45 kDa
• Gene Name: POU class 5 homeobox 1
• Database Link:
  Entrez Gene 5460 Human
  UniProt ID Q01860
• Background: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
• Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding transcription factor 3, Oct-3, Oct-4