Osteoprotegerin (TNFRSF11B) Mouse Monoclonal Antibody [Clone ID: 5G2]

CAT#: AM06539SU-N

Osteoprotegerin (TNFRSF11B) mouse monoclonal antibody, clone 5G2, Ascites

Product Images

Western blot analysis using TNFRSF11B antibody Cat.-No AM06539SU-N against HEK293 (1) and TNFRSF11B (AA: 22-401)-hIgGFc transfected HEK293 (2) cell lysate.

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  Immunofluorescence analysis of HL-60 cells using TNFRSF11B antibody Cat.-No AM06539SU-N (green). Blue: DRAQ5 fluorescent DNA dye.

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  Flow Cytometric analysis of HL-60 cells using TNFRSF11B antibody Cat.-No AM06539SU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 5G2
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow Cytometry: 1/200 - 1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human TNFRSF11B expressed in E. Coli.
• Specificity: Recognizes Osteoprotegerin / TNFRSF11B
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60 kDa
• Gene Name: tumor necrosis factor receptor superfamily member 11b
• Database Link:
  Entrez Gene 4982 Human
  UniProt ID O00300
• Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.
• Synonyms: OPG, OCIF