Estrogen Receptor 1 (ESR1) Mouse Monoclonal Antibody [Clone ID: 6B6]

CAT#: AM06510SU-N

Estrogen Receptor 1 (ESR1) mouse monoclonal antibody, clone 6B6, Ascites

Product Images

Western blot analysis using ESR1 antibody Cat.-No AM06510SU-N against MCF-7 (1), T47D (2) and SKBR3 (3) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using ESR1 antibody Cat.-No AM06510SU-N with DAB staining.

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  Immunofluorescence analysis of Hela cells using ESR1 antibody Cat.-No AM06510SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Product Data

• Clone Name: 6B6
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human ESR1 expressed in E. Coli.
• Specificity: Recognizes ESR1
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 66 kDa
• Gene Name: estrogen receptor 1
• Database Link:
  Entrez Gene 2099 Human
  UniProt ID P03372
• Background: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1