BCL2 Mouse Monoclonal Antibody [Clone ID: 8E12]

CAT#: AM06485SU-N

BCL2 mouse monoclonal antibody, clone 8E12, Ascites

Product images

Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) using BCL-2 mouse mAb with DAB staining.

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  Immunofluorescence analysis of NIH/3T3 cells using BCL-2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow cytometric analysis of 3T3L1 cells using BCL-2 mouse mAb (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 8E12
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH.
• Specificity: This antibody reacts to BCL-2.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 26 kDa
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Background: This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Tissue specificity: Expressed in a variety of tissues.
• Synonyms: BCL2, Bcl-2 alpha