Integrin alpha 5 (ITGA5) Mouse Monoclonal Antibody [Clone ID: 10F6]

CAT#: AM06388SU-N

Integrin alpha 5 (ITGA5) mouse monoclonal antibody, clone 10F6, Ascites

Product Images

Western blot analysis using ITGA5 mouse mAb against ITGA5-hIgGFc transfected HEK293 cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human Placenta tissues using ITGA5 mouse mAb

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  Flow cytometric analysis of Hela cells using ITGA5 mouse mAb (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 10F6
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human ITGA5 expressed in E. Coli.
• Specificity: This antibody reacts to ITGA5.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 114 kDa
• Gene Name: integrin subunit alpha 5
• Database Link:
  Entrez Gene 3678 Human
  UniProt ID P08648
• Background: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.
• Synonyms: Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA