Eph receptor B1 (EPHB1) Mouse Monoclonal Antibody [Clone ID: 5F10A4]

CAT#: AM06214SU-N

Eph receptor B1 (EPHB1) mouse monoclonal antibody, clone 5F10A4, Ascites

Product Images

Western blot analysis using EhpB1 antibody Cat.-No AM06214SU-N against MDA-MB-468 (Lane 1), MDA-MB-453 (Lane 2), MCF-7 (Lane 3), T47D (Lane 4) and SKBR-3 (Lane 5) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and colon cancer (right) showing cytoplasmic localization with DAB staining using EphB1 antibody Cat.-No AM06214SU-N

Specifications

Product Data

• Clone Name: 5F10A4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blotting: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of EphB1 (aa19-133) expressed in E. Coli.
• Specificity: Recognizes EPH receptor B1 (EhpB1).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: EPH receptor B1
• Database Link:
  Entrez Gene 2047 Human
  UniProt ID P54762
• Background: EhpB1: EPH receptor B1. It is a receptor for ephrin-B family members. Ephrin receptors and their ligands, the ephrins, medate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.
• Synonyms: EPHT2, NET, HEK6, ELK, EPH2, Ephrin type-B receptor 1