LPP Mouse Monoclonal Antibody [Clone ID: 8B3A11]

CAT#: AM06208SU-N

LPP mouse monoclonal antibody, clone 8B3A11, Ascites

Product Images

Western blot analysis using anti-LPP Monoclonal Antibody against Hela (Lane 1), NIH/3T3 (Lane 2), COS (Lane 3), Caki (Lane 4), MCF-7 (Lane 5), HepG2 (Lane 6) and SMMC-7721 (Lane 7) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human vessels tissues using anti-LPP Monoclonal Antibody

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  Immunohistochemical analysis of paraffin-embedded human small intestine using anti-LPP Monoclonal Antibody with DAB staining.

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  Confocal immunofluorescence analysis of COS cells using anti-LPP Monoclonal Antibody (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

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  Confocal immunofluorescence analysis of Hela cells using anti-LPP Monoclonal Antibody (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

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Specifications

Product Data

• Clone Name: 8B3A11
• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blotting: 1/500-1/2000.
  Immunofluorescence: 1/200-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
• Reactivities: Hamster, Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human LPP expressed in E. Coli.
• Specificity: Recognizes LIM Protein / LPP.
• Formulation: State: Ascites
  State: Ascitic fluid
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 66 kDa
• Gene Name: LIM domain containing preferred translocation partner in lipoma
• Database Link:
  Entrez Gene 4026 Human
  UniProt ID Q93052
• Background: LIM domain containing preferred translocation partner in lipoma. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP,TRIP6 and Zyxin. LPP (LIM-containing lipoma-preferred partner), a LIM domain-containing scaffolding protein contains three LIM domains at its carboxyterminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains. LPP, an 80 kDa protein, localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts,and shuttles to the nucleus where it has transcriptional activation capacity.The human LPP gene maps to chromosomal location 3q28, and preferentiallytranslocates to the HMGIC gene in a subclass of human benign mesenchymal tumors known as lipomas.
• Synonyms: LPP, Lipoma-preferred partner