Cytokeratin 5 (KRT5) Mouse Monoclonal Antibody [Clone ID: 3E2F1]

CAT#: AM06179SU-N

Cytokeratin 5 (KRT5) mouse monoclonal antibody, clone 3E2F1, Ascites

Product Images

Western blot analysis using Cytokeratin 5 antibody Cat.-No AM06179SU-N against truncated CK5 recombinant protein

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  Immunohistochemical analysis of paraffin-embedded human skin tissues using Cytokeratin 5 antibody Cat.-No AM06179SU-N

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  Immunohistochemical analysis of paraffin-embedded human esophagus epithelium (A), salivary gland basal cell (B), lung squamous cell carcinoma (C), endometrium admosquamous carcinoma (D), showing cytoplasmic and membrane localization using Cytokeratin 5 antibody Cat.-No AM06179SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 3E2F1
• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Bloting: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of CK5 expressed in E. Coli.
• Specificity: Recognizes Cytokeratin 5
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: keratin 5
• Database Link:
  Entrez Gene 3852 Human
  UniProt ID P13647
• Background: CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.
• Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5