LCK Mouse Monoclonal Antibody [Clone ID: 8E5F9]

CAT#: AM06133SU-N

LCK mouse monoclonal antibody, clone 8E5F9, Ascites

Product Images

Western blot analysis using LCK antibody Cat.-No AM06133SU-N against MOLT-4 (Lane 1), CCRF-CEM (Lane 2), CCRF-HSB-2 (Lane 3) and Jurkat (Lane 4) cell lysate.

Specifications

Product Data

• Clone Name: 8E5F9
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human Lck expressed in E. Coli.
• Specificity: Recognizes Lck (lymphocyte-specific protein tyrosine kinase)
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 56 kDa
• Gene Name: LCK proto-oncogene, Src family tyrosine kinase
• Database Link:
  Entrez Gene 3932 Human
  UniProt ID P06239
• Background: Lck (lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.
• Synonyms: p56-LCK, LSK