KDM1A Mouse Monoclonal Antibody [Clone ID: 1B2E5]

CAT#: AM06113SU-N

KDM1A mouse monoclonal antibody, clone 1B2E5, Ascites

Product Images

Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and kidney carcinoma (right), showing nuclear localization using LSD1 mouse mAb with DAB staining.

Specifications

Product Data

• Clone Name: 1B2E5
• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/2000.
  Immunofluorescence: 1/200-1/1000.
  ELISA: 1/10000.
  Immunohistochemistry on Paraffin Sections: 1/200- 1/1000.
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human LSD1 expressed in E. Coli.
• Specificity: This antibody reacts to LSD1/AOF2.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 93 kDa
• Gene Name: lysine demethylase 1A
• Database Link:
  Entrez Gene 23028 Human
  UniProt ID O60341
• Background: The amine oxidase domain 2 (AOF2) gene encodes a nuclear protein (LSD1, ~95kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serve as a docking module for the stabilization of the associated corepressor complex (es) on chromatin.
• Synonyms: Lysine-specific histone demethylase 1, KIAA0601, LSD1