Siglec1 Rat Monoclonal Antibody [Clone ID: 3D6.112]

CAT#: AM05902PU-N

Siglec1 rat monoclonal antibody, clone 3D6.112, Purified

Product Images

Frozen Mouse spleen stained with Rat anti Mouse CD169 Antibody (Clone 3D6.112)

Specifications

Product Data

• Clone Name: 3D6.112
• Applications: FC, IF, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1/50-1/100 (Fixation with either 2% paraformaldehyde or ethanol is recommended).
  Flow Cytometry: 1/100-1/1000.
  Immunocytochemistry.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Sialoadhesin.
  Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
• Specificity: This antibody recognises Mouse CD169, a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Should this product contain a precipitate we recommend microcentrifugation before use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: sialic acid binding Ig-like lectin 1, sialoadhesin
• Database Link:
  Entrez Gene 20612 Mouse
  UniProt ID Q62230
• Background: Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
• Synonyms: Sialoadhesin, Siglec-1