SLA CLASS II DR Mouse Monoclonal Antibody [Clone ID: 2E9/13]

CAT#: AM05887PU-S

SLA CLASS II DR mouse monoclonal antibody, clone 2E9/13, Purified

Product Images

Pig peripheral blood lymphocytes stained with SLA Class II DR Antibody Cat.-No AM05887PU-S (Clone 2E9/13) followed by Goat anti Mouse IgG -FITC Cat.-No SP1041F

Specifications

Product Data

• Clone Name: 2E9/13
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 μl of 1/25-1/200 diluted antibody to label 10⁶ cells in 100 μl.
  Immunoprecipitation: This antibody immunoprecipitates a heterodimer composed of two polypeptides of 28 and 35kD from NP-40 extracts of biotin surface-labelled porcine peripheral blood mononuclear cells.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Bovine, Porcine
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Porcine monocytes.
• Specificity: Clone 2E9/13 is specific for SLA DR molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells.
  This clone reacts with lymphocytes from all outbred and miniature pigs so far tested, suggesting that it recognises a monomorphic determinant of porcine SLA DR. Clone 2E9/13 is reported to inhibit the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B, see Bullido, R. et al. for details.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Background: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.
• Synonyms: MHC Class II DR