P Glycoprotein (ABCB1) Mouse Monoclonal Antibody [Clone ID: UIC2]

CAT#: AM05632PU-L

P Glycoprotein (ABCB1) mouse monoclonal antibody, clone UIC2, Purified

Specifications

Product Data

• Clone Name: UIC2
• Applications: FC, IF, IHC, IP
• Recommended Dilution: Immunoprecipitation.
  Immunofluorescence.
  Flow Cytometry: Use 10 µl of neat MDR1/CD243 antibody to label 1x10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/200. This product may benefit from antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse Balb/c 3T3 fibroblasts transfected with Human CD243 cDNA.
• Specificity: This antibody specifically recognises an extracellular conformational epitope of CD243, also known as MDR1 (multidrug resistance protein 1) and Pgp (Pglycoprotein),
  a transmembrane protein and member of the ABC transporter (ATPbinding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds.
  Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell. Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase.
  We recommend the use of AM05632LE-N for Functional Studies.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ATP binding cassette subfamily B member 1
• Database Link:
  Entrez Gene 5243 Human
  UniProt ID P08183
• Background: CD243 is also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds.
  CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs.
• Synonyms: Multidrug resistance protein 1, P-glycoprotein 1, ABCB1, PGY1