MMP2 Mouse Monoclonal Antibody [Clone ID: 4D3]

CAT#: AM05377PU-N

MMP2 mouse monoclonal antibody, clone 4D3, Purified

Product Images

Left: Immunohistochemical staining of human oesophageal adenocarcinoma tissue using MMP-2 antibody. Right: Western blot using MMP-2 antibody on recombinant MMP-2 proenzyme (400 ng/lane).

Specifications

Product Data

• Clone Name: 4D3
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: 1 - 2 µg/ml.
  Immunohistochemistry on paraffin sections: 1 - 5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the Cterminus of the human MMP2 protein and mouse myeloma Ag8563 cells.
• Specificity: This antibody reacts to Matrix Metalloproteinase 2.
• Formulation: Phosphate buffered saline with 0.08% sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20°C only.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: matrix metallopeptidase 2
• Database Link:
  Entrez Gene 4313 Human
  UniProt ID P08253
• Background: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves Collagen Types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.
• Synonyms: MMP2, CLG4A, 72 kDa gelatinase, Matrix metalloproteinase-2, Gelatinase A, TBE-1