p53 (TP53) (371-380) Mouse Monoclonal Antibody [Clone ID: HR231]

CAT#: AM05327PU-N

p53 (TP53) (371-380) mouse monoclonal antibody, clone HR231, Purified

Product Images

Figure 1. Western blot analysis using p53 antibody at 1 ug/ml on native H1299 cells (Lane 1) and H1299 cells transfected with human p53 (Lane 2).

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  Figure 2. Graphic representation of the epitope location.

Specifications

Product Data

• Clone Name: HR231
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Western Blot: 1 - 5 µg/ml.
  ELISA.
  Immunoprecipitation.
  Immunohistochemistry.
  Positive Control: Breast or colon carcinoma cells.
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from immunized with full length human p53 protein and mouse myeloma cells.
• Specificity: This antibody reacts to p53.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction.
  Preservative: 0.08% Sodium Azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A/G
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: tumor protein p53
• Database Link:
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13