Myog Mouse Monoclonal Antibody [Clone ID: F12B]

CAT#: AM05309PU-N

Myog mouse monoclonal antibody, clone F12B, Purified

Product Images

Figure 1. Immunohistochemical staining using myogenin antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma.

Specifications

Product Data

• Clone Name: F12B
• Applications: ELISA, EMSA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Gel Supershift.
  Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 µg/ml).
  Immunofluorescence.
  Immunoprecipitation (2 µg/ml).
  Western Blot (1 µg/ml).
  Positive Control: Rhabdomyosarcoma and Wlim's tumors.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
• Specificity: Recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
  This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.
• Formulation: PBS containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: myogenin
• Database Link:
  Entrez Gene 4656 HumanEntrez Gene 17928 MouseEntrez Gene 29148 Rat
  UniProt ID P20428
• Background: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
• Synonyms: MYOG, MYF4, Myogenic factor 4, BHLHC3