ICAM1 Mouse Monoclonal Antibody [Clone ID: 1H4]

CAT#: AM03205PP-N

ICAM1 mouse monoclonal antibody, clone 1H4, PerCP

Specifications

Product Data

• Clone Name: 1H4
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Raji cells and spleen cells fused with NS1 cells
• Specificity: The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
• Formulation: Phosphate buffered saline (PBS)
  Label: PerCP
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA)
  Preservative: 15 mM sodium azide
  Label: Conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PerCP
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This products is photosensitive and should be protected from light.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: intercellular adhesion molecule 1
• Database Link:
  Entrez Gene 3383 Human
  UniProt ID P05362
• Background: CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
• Synonyms: ICAM-1