Acetylated Lysine Mouse Monoclonal Antibody [Clone ID: 7F8]

CAT#: AM03158PU-N

Acetylated Lysine mouse monoclonal antibody, clone 7F8, Purified

Specifications

Product Data

• Clone Name: 7F8
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry.
  Western blot: 1 µg/ml was sufficient for detection of 20 µg Acetylated Chicken Erythrocyte Histones.
• Reactivities: Bovine, Birds
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Acetylated KLH
• Specificity: Detects proteins containing Acetylated lysine residues in ELISA and Western Blots.
  Does not detect non-Acetylated lysine residues.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide in 50% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1).
  The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2).
  Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
  The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).
• Synonyms: AcK, acetyl Lysine, acetyl-Lysine