CD105 (ENG) Mouse Monoclonal Antibody [Clone ID: MEM-229]

CAT#: AM03092RP-N

CD105 (ENG) mouse monoclonal antibody, clone MEM-229, PE

Product Images

Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).

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  Surface staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PE.

Specifications

Product Data

• Clone Name: MEM-229
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry analysis of Human blood cells using 20 µl reagent/100 µl of whole blood or 10e6 cells in a suspension.
• Reactivities: Human, Porcine
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Vaccinia virus containing the Human CD105 (L-isoform) cDNA
• Specificity: This antibody recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
  Negative Species: Canine (Dog), Equine (Horse).
• Formulation: Phosphate buffered saline (PBS) containing 15 mM Sodium Azide and 0.2% (w/v) high-grade protease free BSA as a stabilizing agent.
  Label: PE
  State: Liquid purified Ig fraction
  Label: Conjugated with R-Phycoerythrin
• Purification: Size-Exclusion Chromatography
• Conjugation: PE
• Storage: Store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: endoglin
• Database Link:
  Entrez Gene 2022 Human
  UniProt ID P17813
• Background: CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbR-2 as a receptor for TGFb-1 and TGFb-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFb-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
• Synonyms: ENG, END, HHT1, ORW, ORW1
• Note: The content of a vial (2 ml) is sufficient for 25 tests.