CD105 (ENG) Mouse Monoclonal Antibody [Clone ID: MEM-229]

CAT#: AM03092PU-N

CD105 (ENG) mouse monoclonal antibody, clone MEM-229, Purified

Product Images

Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue). Primary antibody dilution: 5 ug/ml.

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  Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).

Specifications

Product Data

• Clone Name: MEM-229
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: 10 μg/ml.
  Positive Control: Kg1 Human acute myelogenous leukemia cell line.
  Western Blotting (Non-Reducing Conditions).
  Immunocytochemistry.
  Immunohistochemistry on Frozen Sections: 1/200 (Acetone Fixation).
• Reactivities: Human, Porcine
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Vaccinia virus containing the Human CD105 (L-isoform) cDNA.
• Specificity: The antibody MEM-229 recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Protein-A Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: endoglin
• Database Link:
  Entrez Gene 2022 Human
  UniProt ID P17813
• Background: CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFβR-2 as a receptor for TGFβ-1 and TGFβ-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFβ-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
• Synonyms: ENG, END, HHT1, ORW, ORW1