Factor I (CFI) Mouse Monoclonal Antibody [Clone ID: 3R/8]

CAT#: AM01335PU-N

Factor I (CFI) mouse monoclonal antibody, clone 3R/8, Purified

Specifications

Product Data

• Clone Name: 3R/8
• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: ELISA: 1/3000 - 1/4000.
  Western Blot.
  Functional Assays.
  Immunohistochemistry on frozen sections.
  Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Native human factor I from serum.
• Specificity: This antibody recognises complement factor I, a member of the peptidase S1 family present in the blood plasma.
• Formulation: Borate buffered saline pH 8.4 containing 0.02% Sodium Azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: complement factor I
• Database Link:
  Entrez Gene 3426 Human
  UniProt ID P05156
• Background: Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors. Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome.
• Synonyms: CFI, IF, KAF, AHUS3, C3BINA, C3b-INA
• Note: This antibody blocks function of complement factor I. Removal of Sodium Azide is recommended prior to use in functional assays.