CD79A (202-216) Mouse Monoclonal Antibody [Clone ID: HM57]

CAT#: AM01322PU-S

CD79A (202-216) mouse monoclonal antibody, clone HM57, Purified

Product Images

Staining of paraffin embedded human tonsil with mouse anti human CD79a antibody.

Specifications

Product Data

• Clone Name: HM57
• Applications: FC, IHC, WB
• Recommended Dilution: Western blot.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/100-1/500. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
  Recommended Positive Control: Human tonsil.
  Flow Cytometry: Use 10 µl of 1/10-1/50 diluted CD79a antibody to label 1x10⁶ cells in 100 µl (Membrane permeabilisation is required).
• Reactivities: Bison, Bovine, Canine, Chicken, Deer, Equine, Guinea Pig, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of Human mb-1.
• Specificity: This antibody recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD79a molecule
• Database Link:
  Entrez Gene 973 Human
  UniProt ID P11912
• Background: The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin.
• Synonyms: IGA, MB1, B-Cell marker