Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: Ki-67]
Ki67 (MKI67) mouse monoclonal antibody, clone Ki-67, Purified
|Applications||FC, IF, WB|
|Recommended Dilution||Flow cytometry (Intracellular staining).
Immunocytochemistry: 1 μg/ml (Paraformaldehyde fixation).
|Immunogen||Nuclei of the Hodgkin lymphoma cell line L428|
|Specificity||This antibody recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.|
|Formulation||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State: Liquid Ig fraction
|Purification||Protein-A affinity chromatography (> 95% pure by SDS-PAGE)|
|Storage||Store undiluted at 2-8°C.
DO NOT FREEZE!
|Stability||Shelf life: one year from despatch.|
|Background||Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.|
|Synonyms||Ki67 antigen, MKI67, Proliferation marker|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.