Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: Ki-67]

CAT#: AM01167PU-N

Ki67 (MKI67) mouse monoclonal antibody, clone Ki-67, Purified

Product Images

Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).

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  Flow cytometry analysis of human peripheral blood mononuclear cells stimulated with PHA. Surface staining of CD25 (clone MEM-181 APC) was followed by permeabilization and nuclear staining of Ki-67 (clone Ki-67 PE).

Specifications

Product Data

• Clone Name: Ki-67
• Applications: FC, IF, WB
• Recommended Dilution: Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining.<br>
  Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 μg/ml.
• Reactivities: Bovine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Nuclei of the Hodgkin lymphoma cell line L428
• Specificity: This antibody recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: marker of proliferation Ki-67
• Database Link:
  Entrez Gene 4288 Human
  UniProt ID P46013
• Background: Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
• Synonyms: Ki67 antigen, MKI67, Proliferation marker