Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: Ki-67]

CAT#: AM01167PU-N

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Ki67 (MKI67) mouse monoclonal antibody, clone Ki-67, Purified



USD 220.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Clone Name Ki-67
Applications FC, IF, WB
Recommended Dilution Flow cytometry (Intracellular staining).
Western blot.
Immunohistochemistry.
Immunocytochemistry: 1 μg/ml (Paraformaldehyde fixation).
Reactivity Bovine, Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Nuclei of the Hodgkin lymphoma cell line L428
Specificity This antibody recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Formulation Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State: Purified
State: Liquid Ig fraction
Concentration lot specific
Purification Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Background Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Synonyms Ki67 antigen, MKI67, Proliferation marker
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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