pan Dengue Virus Mouse Monoclonal Antibody [Clone ID: Dengue 1-11(3)]

CAT#: AM01108PU-N

pan Dengue Virus mouse monoclonal antibody, clone Dengue 1-11(3), Purified

Specifications

Product Data

• Clone Name: Dengue 1-11(3)
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot (Reducing Conditions).
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: Requires antigen retrieval using heat treatment prior to staining of paraffin Sections (Sodium citrate buffer pH 6.0 is recommended for this purpose).
• Reactivities: Dengue Virus Types 1, Dengue Virus Types 2, Dengue Virus Types 3, Dengue Virus Types 4
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recognizes all four dengue virus serotypes (DEN-1, DEN-2, DEN-3 and DEN-4) of the genus Flavivirus.
  Clone Dengue 1-11 (3) binds strongly to subtypes 1 and 2. It recognizes subtype 3 more weakly. Among the four subtypes, subtype 4 is recognized the least strongly and some customers have reported no recognition of subtype 4.
  The antibody detects an anti-E/envelope specific band of 61kDa under Reducing Conditions in Western blotting. A weak secondary band of 80kDa may also be apparent.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: The dengue virus is responsible for the tropical and sub-tropical diseases, dengue (DF) and dengue haemorrhagic fever (DHF), transmitted to individuals through the bite of the Aedes mosquito. The global distribution of dengue is expanding and comparable to that of malaria, with symptoms ranging from a mild viral syndrome of high fever, rash, headache, fatigue and muscle and joint pain, to the more severe and sometimes fatal DHF. Each dengue virus serotype is antigenically distinct, such that infection only provides an individual with immunity to the causative serotype, with the possibility of further infection by a different serotype or even multiple serotypes.