CD22 Mouse Monoclonal Antibody [Clone ID: RFB-4]

CAT#: AM00647RP-N

CD22 mouse monoclonal antibody, clone RFB-4, PE

Specifications

Product Data

• Clone Name: RFB-4
• Applications: IF, IHC
• Recommended Dilution:

  Flow Cytometry: 5 μl is the maximum amount of reagent required for 1 x 10⁶ cells. Lesser amounts of antibody may be sufficient and it is recommended that the customer determine the optimum amount of antibody for each application.
  Immunohistochemistry on Frozen Sections: Staining of frozen sections by direct fluorescence or by immuno-chemical techniques.
  Quantitative determination of peripheral B-cells (CD22⁺) in blood.
  Identification of CD22⁺ -cells in tissue sections.
  Determination of B-cell origin of lymphoid neoplasms.
  Quantitative elimination or isolation of B-cells by flow cytometry or magnet beads.

• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody reacts with the CD22 antigen. CD22 is a heterodimer consisting out of 2 glycoproteins (130 and 140 kD).
  Antigen Distribution:
  Peripheral blood lymphocytes: 12±3%
  T-cells (E⁺): < 1%
  B-cells (E^-, Ig⁺): 92±3%
  Monocytes (CD14⁺): < 1%
  Granulocytes: < 1%
  Thymocytes: < 1%
• Formulation: Label: PE
  State: Liquid purified IgG fraction
  Stabilizer: A highly purified grade of BSA has been added as a stabilizing protein to bring the final protein concentration to 4-5 mg/ml
  Preservative: Sodium Azide
  Molar radio: F/P :1
• Concentration: lot specific
• Conjugation: PE
• Storage: Store the antibody undiluted in the dark at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: CD22 molecule
• Database Link:
  Entrez Gene 933 Human
  UniProt ID P20273
• Background: CD22 is a heterodimer consisting out of 2 glycoproteins (130 and 140 kD). The molecule is involved in signal transduction in B-cells activated via surface immunoglobulines. CD22 is in principle a B lineage antigen, that is present in the cytoplasm of progenitor B-cells and on the membrane of the majority of mature peripheral B-lymphocytes. Fur-thermore, it is strongly expressed on Hairy Cell Leukemia cells and very weakly on some other leukemias.
• Synonyms: SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM