Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: PY20]

CAT#: AM00199TC-S

Phosphotyrosine mouse monoclonal antibody, clone PY20, TAMRA

Specifications

Product Data

• Clone Name: PY20
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Suitable for Immunohistochemistry (5-10 ug/ml) and Immunocytochemistry (5-10 ug/ml).
• Reactivities: Broad
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
• Specificity: This antibody specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
• Formulation: 0.02 M Sodium Phosphate, pH 7.5 with 0.15 M Sodium Chloride, 3 mM Sodium Azide as preservative, 50% glycerol
  Label: TAMRA
  State: Liquid purified IgG fraction.
  Label: The antibody was labeled by reaction with tetramethyl-rhodamine succinimidyl ester in the presence of phenyl phosphate (to protect the antibody binding site). The unreacted succinimidyl ester was removed by molecular exclusion chromatography
• Concentration: lot specific
• Purification: Affinity purification on a column of immobilized phosphotyrosine.
• Conjugation: TAMRA
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Background: Phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation. Its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function.
  The rhodamine conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.