Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: PY20]

CAT#: AM00199HR-T

Phosphotyrosine mouse monoclonal antibody, clone PY20, HRP

Specifications

Product Data

• Clone Name: PY20
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: Suitable for Western Blot, ELISA, Immunohistochemistry and Immunocytochemistry.
  Working dilution of 1/2,000 is suggested for Western blot or ELISA, 1/500 for Immunohistochemistry or Immunocytochemistry.
• Reactivities: Broad
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
• Specificity: Clone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
• Formulation: 50% glycerol, 0.02 M sodium phosphate, pH 7.5, 0.15 M sodium chloride.
  Label: HRP
  State: Liquid purified IgG fraction
  Label: Coupled through free primary amino groups to Periodate-activated Horseradish Peroxidase The binding site of the antibody was protected by the addition of phenyl phosphate prior to coupling
• Concentration: lot specific
• Purification: Affinity purification on a column of immobilized phosphotyrosine
• Conjugation: HRP
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Background: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years, While the phosphorylation of specific tyrosine residues has be shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved invaluable to the study of tyrosine-phosphorylated proteins and the biochemical pathways in which they fuction. The HRP conjugate of PY20 anti-phosphotyrosine is especailly useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immuncytochemical protcols in situations wherin the use of a secondary antibody would produce undesirable background signals.