SHC (SHC1) pTyr239/240 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1E3]

CAT#: AM00141PU-N

SHC (SHC1) pTyr239/240 (incl. pos. control) mouse monoclonal antibody, clone 1E3, Purified

Product Images

Phosphospecificity Whole cell extracts of control (co), EGF-stimulated (EGF) or pervanadate-treated (VH) A549 tumor cells were applied to SDS-PAGE (ca. 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-1E3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

Specifications

Product Data

• Clone Name: 1E3
• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.05 µg/ml.
  Immunohistochemistry.
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH
• Specificity: This antibody specifically recognizes shc when it is phosphorylated at Tyr 239/Tyr 240.
• Formulation: 1 ml PBS / 0.09 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: SHC adaptor protein 1
• Database Link:
  Entrez Gene 6464 Human
  UniProt ID P29353
• Background: Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signaling pathways.
• Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3, SHCA