p38 (MAPK14) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 13D5]

CAT#: AM00136PU-N

p38 (MAPK14) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 13D5, Purified

Product Images

Detection of endogenous SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK2a-13D5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D

Specifications

Product Data

• Clone Name: 13D5
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: Western Blot: 1 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.05 µg/ml.
  Immunoprecipitation: 1 - 10 µg per 106 pervanadate-treated A431 cells.
  Immunocytochemistry.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH
• Specificity: This antibody specifically interacts with the N-terminus of SAPK2 / p38 / CSBP.
• Formulation: 1 ml PBS / 0.1 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: mitogen-activated protein kinase 14
• Database Link:
  Entrez Gene 1432 Human
  UniProt ID Q16539
• Background: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. Stress-activated Protein Kinase 2 (SAPK2/p38) phosphorylates conserved Ser - Pro or Thr - Pro peptide motifs.
• Synonyms: Mitogen-activated protein kinase 14, p38 alpha, MXI2, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1
• Note: Molecular Weight: 38kDa.
  Epitope: N-terminus, interacts with SAPK2 (p38/CSBP).