MEK5 (MAP2K5) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14B5]

CAT#: AM00095PU-N

MEK5 (MAP2K5) (incl. pos. control) mouse monoclonal antibody, clone 14B5, Purified

Product Images

Detection of endogenous MKK5 Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK5-14B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2

Specifications

Product Data

• Clone Name: 14B5
• Applications: WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Positive Control: Cell lysate from untreated A431 cells.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to hemocyanin
• Specificity: This antibody reacts to MKK5.
• Formulation: 1 ml PBS / 0.09 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: mitogen-activated protein kinase kinase 5
• Database Link:
  Entrez Gene 5607 Human
  UniProt ID Q13163
• Background: Mitogen-activated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK subfamily (MAP2K, MKK or MEKs). MKK5 specifically phosphorylates erk5/MAPK7 within the kinase activation loop pThr-E-pTyr motif. After activation erk5 phosphorylates MKK5 on multiple phosphoserine residues. The MKK5/erk5 pathway may play an important role in prostate and breast cancer.
• Synonyms: MAP kinase kinase 5, MAPKK 5, MEK5, MKK5