HSP27 (HSPB1) pSer82 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5B9]

CAT#: AM00070BT-N

HSP27 (HSPB1) pSer82 (incl. pos. control) mouse monoclonal antibody, clone 5B9, Biotin

Specifications

Product Data

• Clone Name: 5B9
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Positive Control: Cell lysate from EGF-treated HepG2 cells.
  Immunohistochemistry on frozen sections.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH
• Specificity: This antibody specifically interacts with hsp27 phosphorylated at serine 82 and does not crossreact with the non-phosphorylated hsp27 nor with unrelated serine-phosphorylated proteins.
• Formulation: PBS/0.09% Na-Azide/PEG and Sucrose
  Label: Biotin
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Size exclusion chromatography
• Conjugation: Biotin
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: heat shock protein family B (small) member 1
• Database Link:
  Entrez Gene 3315 Human
  UniProt ID P04792
• Background: The small heat shock protein hsp27 is constitutively expressed in most cell lines. The expression level is increased in response to environmental stress. Activation of the p38 kinases results in subsequent activation of MAPKAP2 that phosphorylates hsp27 at Ser15, Ser78 and Ser82.
• Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25