c Fos (FOS) pSer374 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 34E4]

CAT#: AM00061PU-N

c Fos (FOS) pSer374 (incl. pos. control) mouse monoclonal antibody, clone 34E4, Purified

Product Images

fos activation Serum starved HepG2 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). Immunoblots were probed with mab fos-34E4 (0.5 ug/ ml) for 1h at room temperature and developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF lane 7: 4h EGF; lane 8: 8h EGF

Specifications

Product Data

• Clone Name: 34E4
• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.1 µg/ml (protein ELISA).
  Immunohistochemistry on frozen sections.
• Reactivities: Canine, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
• Specificity: This antibody specifically interacts with c-Fos phosphorylated at serine 374.
• Formulation: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch
• Gene Name: Fos proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  Entrez Gene 2353 Human
  UniProt ID P01100
• Background: The immediated early gene product c-Fos is expressed following mitogenic stimulation. c-Fos functions as a sensor for MAPK signal duration. When MAPK activation is transient, MAPK activity declines before accumulation of the c-Fos protein. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at serine 374. Phosphorylation stabilizes the Fos protein and primes c-Fos for additional phosphorylation at threonine 325.
• Synonyms: FOS, G0S7