Her2 (ERBB2) (860-880) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24B5]

CAT#: AM00049PU-N

Her2 (ERBB2) (860-880) (incl. pos. control) mouse monoclonal antibody, clone 24B5, Purified

Product Images

Detection of endogenous erbB2 Whole cell extracts of serum starved, EGF-treated (15 min) tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB2-24B5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2A 549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT Lane 6: PC3 Lane 7: HeLa Lane 8: HepG2

Specifications

Product Data

• Clone Name: 24B5
• Applications: WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  This product contains a Positive Control for Immunoblot Applications (for details see "Protocols")
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic petide conjugated to KLH.
  Epitope: Intracellular domain (aa 860-880)
• Specificity: This antibody specifically recognizes the intracellular domain of erbB2.
• Formulation: 1 ml PBS containing 0.09% Sodium Azide, PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 1 ml H₂O (15 min, RT).
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid Nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 185 kDa
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  Entrez Gene 2064 Human
  UniProt ID P04626
• Background: ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248.Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.
• Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19
• Note: Protocol: Positive Control: SKOV-3 Untreated
  Formulation:
  Lyophilized Cell Lysate from Serum starved SKOV-3 Cells.
  Stability:
  Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  Application:
  The Positive Control cell lysate is recommended for immunoblot applications. 20µl of positive control cell lysate corresponds to ca 20.000 cells.
  Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  
  Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
  Storage:
  Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  Shelf life: one year from despatch.