EGFR pTyr1110 Mouse Monoclonal Antibody [Clone ID: 8B8]

CAT#: AM00039PU-N

EGFR pTyr1110 mouse monoclonal antibody, clone 8B8, Purified

Product Images

Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) MDA-MB 468 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-8B8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

Specifications

Product Data

• Clone Name: 8B8
• Applications: ELISA, WB
• Recommended Dilution: Western blot (1 μg/ml for HRPO/ECL detection, recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer).
  ELISA (0.1 µg/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to hemocyanin
  Epitope: phosphotyrosine 1110, N P V pY H N Q
• Specificity: Thisa antibody specifically recognizes EGFR phosphorylated at Tyrosine 1110. It does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins.
• Formulation: PBS, 0.09 % Na-azide, PEG and Sucrose
  State: Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: Reconstitute in 1 ml distilled water for 15 min at RT
• Purification: Subsequent ultrafiltration and size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilised product upon arrival at -20 °C.
  Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze in liquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing.
  Should this product contain a precipitate, we recommend centrifugation before use.
• Stability: Shelf life: One year from despatch.
• Gene Name: epidermal growth factor receptor
• Database Link:
  Entrez Gene 1956 Human
  UniProt ID P00533
• Background: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases.
  Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.
• Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
• Note: Mol. weight: 180 kDa.
  Includes positive control: Cell lysate from pervanadate-treated A431 cells (see protocols).
  
  Protocol: Positive control
  Format: Lyophilized cell lysate from A431 cells. Serum starved cells were treated for 15 min with pervanadate.
  Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90° C for 5 min.
  Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.