Purified RNase A isolated from bovine pancreas. RNase A is an endoribonuclease that exists as a single chain polypeptide containing 4 disulphide bridges. It can be inhibited by alkylation of the histidine-12 or histidine-119 that are present in the active site. Activators of RNase A include potassium and sodium salts. The optimal conditions for activity are 60°C and pH 7.6.
Prepared from bovine nasal cartilage and is free from other collagens, serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collage Type II. Reaction with anti-Collagen I, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).