This protein is a preparation of human tenascin purified by precipitation, concentration and dialysis from tissue culture supernatant of the glioblastoma U-251 MG, formally distributed as U-373 MG, an astrocytoid adherent cell line, an over producer of human tenascin.
Horseradish Peroxidase (HRP) conjugated Protein A. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G
Preparation: Collagens were extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation. Thermal denaturation converts the collagen to gelatin.
3 x Flag Peptide. This peptide represents a sequence of triple DYKDDDDK epitopes that is used in studying the function and structure of proteins. It has an increased sensitivity to the anti- DYKDDDDK M2 antibody and can be detected at lower abundance than the octomeric DYKDDDDK tag.