Gene Expression

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Camk2d - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro

Camk2d CRISPRa kit - CRISPR gene activation of mouse calcium/calmodulin-dependent protein kinase II, delta

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

qSTAR qPCR primer pairs against Mus musculus gene Camk2d

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

qPCR primer pairs and template standards against Mus musculus gene Camk2d

Application Plasmid of exact quantity for transcript copy number calculation