Antibodies

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Equine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/1,000-1/10,000), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/200,000 of the reconstitution concentration is suggested for this product.
Reactivities Horse
Conjugation HRP

Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot: use at 1/1,000-1/5,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (use at 1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Reactivities Horse
Conjugation HRP

Equine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western blot: 1/5,000-1/20,000 or Dot blot), ELISA (1/50,000-1/200,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Horse
Conjugation HRP

Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Immunofluorescence: 1/500 - 1/2,500.
Flow Cytometry: 1/2,000 - 1/10,000.
Reactivities Horse
Conjugation HRP