Antibodies

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mIgG (1,2a,2b,3) Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9F11

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse, Rat
Conjugation Unconjugated

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified

Applications ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Rat
Conjugation Unconjugated

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE

Applications Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
FLISA: < / = 1.0 µg/ml
Reactivities Rat
Conjugation PE

Human IgG1 rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human, Mouse, Rat
Conjugation Unconjugated

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/100,000.
Western blot: 1/500-1/3,000.
Immunohistochemistry: 1/500-1/1,500.
Reactivities Rat
Conjugation HRP

Rat IgG2b (subclass specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rat
Conjugation Biotin

Rat IgM Mu chain specific F(ab’)2 Fragment mouse monoclonal antibody, clone M 2A1, Purified

Applications B Cell Stimulation: 5 µg per mL of tissue culture media.
Reactivities Rat
Conjugation Unconjugated

Rat IgE (Fc specific) goat polyclonal antibody, Biotin

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Rat
Conjugation Biotin

Rat IgE (Fc specific) goat polyclonal antibody, HRP

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Reactivities Rat
Conjugation HRP

Rat IgG2b (subclass specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in Rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities Rat
Conjugation HRP

Rat IgG2ab (subclass specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000 - 1/10,000.
Reactivities Rat
Conjugation HRP

Rat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/170,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,500.
Reactivities Rat
Conjugation HRP

Rat IgG Antibody (Min X Ms)

Applications WB: 1:10,000 - 1:40,000
IHC: 1:5,000 - 1:20,000
ELISA: 1:20,000 - 1:110,000
Reactivities Rat
Conjugation Unconjugated

F(ab')2 Rat IgG (H&L) Antibody Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Rat
Conjugation Unconjugated