Antibodies

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Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western blot: 1/2,000-1/10,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Human IgG1 rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human, Mouse, Rat
Conjugation Unconjugated

Mouse IgD (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgD in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgD from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
Antibody titre: Precipitin titre 1/32 when tested against monoclonal IgD containing mouse serum in agarblock immunodiffusion titration.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgE (Fc specific) goat polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgE in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
Antibody titre: Precipitin titre 1/32 when tested against monoclonal IgE containing mouse serum in agarblock immunodiffusion titration.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/400,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2b Antibody Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IHC: 1:2,000 - 1:10,000
ELISA: 1:40,000 - 1:200,000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG3 Antibody Pre-adsorbed

Applications WB: 1:4,000 - 1:20,000
IHC: 1:2,000 - 1:10,000
ELISA: 1:40,000 - 1:200,000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H&L) Antibody Pre-adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000 - 1:100,000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (mu chain) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,400 - 1:40,400
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H&L) Antibody Pre-Adsorbed

Applications WB: 1:10,000 - 1:50,000
IHC: 1:5,000 - 1:25,000
ELISA: 1:100,000 - 1:500,000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled secondary antibody for indirect detection of IgE in mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
Immunodiffusion.
Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/40,000-1/180,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Purified

Applications Flow Cytometry: Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA
FLISA
Immunohistochemistry (frozen sections).
Reactivities Mouse
Conjugation Unconjugated