Antibodies

Jump to Filters
View as table Download
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795PU-N

USD 280.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution).
Western blot (1/5000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Immunoprecipitation (1/250).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation Unconjugated
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
PrecipHen® (Chicken IgY) Goat Polyclonal Antibody
AP31795AG-N

USD 670.00

2 Weeks

PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose

Applications Immunoprecipitation.
Reactivities Chicken
Conjugation Agarose
Immunogen Immune sera was collected from goats that were hyper-immunized with purified chicken IgY.
Production: Goat anti-Chicken IgY antibodies were affinity purified from the sera over a Chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated Agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50mM HEPES buffer (pH 7.6) with 5mM EDTA (TE buffer). Sodium Azide was then added to a final concentration of 0.02% (w/v).