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Collagen I (COL1A1) rabbit polyclonal antibody, Biotin
| Applications | ELISA, FC, IHC, IP, WB |
| Reactivities | Bovine, Human, Mammalian, Mouse, Rat |
| Conjugation | Biotin |
| Immunogen | Collagen Type I from Human and Bovine placenta. |
Antibodies
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Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/5,000-1/20,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins. |
Rabbit polyclonal Nag-1 antibody Biotin Conjugated
| Applications | IHC, WB |
| Reactivities | Human, Mouse |
| Conjugation | Biotin |
| Immunogen | Nag-1 Antibody Biotin Conjugated antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH. |
Mouse Kappa Light Chain (free and bound) goat polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, WB |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Alb rabbit polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, R, WB |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Albumin is isolated from Mouse serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
C3 goat polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, IP, WB |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | C3c isolated and purified from pooled normal Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Collagen II (COL2A1) rabbit polyclonal antibody, Biotin
| Applications | ELISA, IHC, IP, WB |
| Reactivities | Bovine, Human, Mouse, Rat, Sheep |
| Conjugation | Biotin |
| Immunogen | Collagen type II purified from adult Human knee and Bovine nasal cartilage. For immunization collagens extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein. |
Mouse IgG (Fc specific) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/20,000-1/100,000 Western blot: 1/2,000-1/10,000 Immunohistochemistry: 1/1,000-1/5,000. Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Anti-mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat. |
Fibrinogen goat polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, IP, WB |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Fibrinogen is isolated from fresh plasma after removing Prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG3 (subclass specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1.000 - 1/5,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Pools of purified homogenous IgG3 isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/5000-1/20000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins. |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100- 1/2500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG (H&L) Antibody Biotin Conjugated
| Applications | WB: 1:2,000 - 1:20,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:350,000 |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat. |
Mouse IgG (H&L) Secondary Antibody Biotin Conjugated
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:200,000 - 1:300,000 |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Mouse IgG whole molecule |
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