Antibodies

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Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494HR-N

USD 705.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, HRP

Applications This antibody can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates To demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in Mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical/Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/7,000 
depending on the method used. Working dilutions schould be stored at +4°C, not refrozen, and preferably used the same day.
Reactivities Mouse
Conjugation HRP
Immunogen Pools of purified homogenous IgG3 isolated from pooled Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Alb Rabbit Polyclonal Antibody
AP31512HR-N

USD 390.00

2 Weeks

Alb rabbit polyclonal antibody, HRP

Applications ELISA, ID, IF, IHC, R, WB
Reactivities Mouse
Conjugation HRP
Immunogen Albumin is isolated from Mouse serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543HR-N

USD 705.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/400.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000 depending on the method used.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992BT-N

USD 705.00

2 Weeks

Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100- 1/2500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992HR-N

USD 705.00

2 Weeks

Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections. 
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions 
Histochemical and cytochemical Use: 1/100- 1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Fibrinogen Goat Polyclonal Antibody
AP21470HR-N

USD 540.00

2 Weeks

Fibrinogen goat polyclonal antibody, HRP

Applications ELISA, ID, IF, IHC, WB
Reactivities Mouse
Conjugation HRP
Immunogen Fibrinogen is isolated from fresh plasma after removing Prothrombin.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgD (Fc specific) Goat Polyclonal Antibody
AP21481FC-N

USD 705.00

2 Weeks

Mouse IgD (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/10-1/40, depending on the method used.
Reactivities Mouse
Conjugation FITC
Immunogen Pools of purified homogenous IgD isolated from BALB/C and C57Bl Mouse serum. F reund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgD (Fc specific) Goat Polyclonal Antibody
AP21481TC-N

USD 540.00

2 Weeks

Mouse IgD (Fc specific) goat polyclonal antibody, TRITC

Applications In immunocytochemical and immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of purified homogenous IgD isolated from BALB/C and C57Bl mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482AF-N

USD 540.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled secondary antibody for indirect detection of IgE in mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Unconjugated
Immunogen Purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482BT-N

USD 540.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgE isolated from Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485FC-N

USD 705.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80, depending on the method used.
Reactivities Mouse
Conjugation FITC
Immunogen Pools of purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485TC-N

USD 705.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, TRITC

Applications Can be used to identify and measure IgE, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of highly purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494AF-N

USD 705.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000.
Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG fraction is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity.
Reactivities Mouse
Conjugation Unconjugated
Immunogen Pools of purified homogenous IgG3 isolated from pooled Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
AP21494TC-N

USD 705.00

2 Weeks

Mouse IgG3 (subclass specific) goat polyclonal antibody, TRITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG3 in mouse serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Working dilutions 1/10 - 1/40, depending on the method used.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of purified homogenous IgG3 isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse Kappa Light Chain (free and bound) Goat Polyclonal Antibody
AP31535HR-N

USD 540.00

2 Weeks

Mouse Kappa Light Chain (free and bound) goat polyclonal antibody, HRP

Applications ELISA, ID, IF, IHC, WB
Reactivities Mouse
Conjugation HRP
Immunogen A pool of purified Bence Jones kappa proteins isolated from mouse urine.
Freund’s complete adjuvant is used in the first step of the immunization procedure.