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Duck IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/4,000. Immunohistochemistry: 1/50-1/250. Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Antibody titre: Precipitin titre 1/16 when tested against pooled normal Duck serum in agar-block immunodiffusion titration. |
| Reactivities | Duck |
| Conjugation | Unconjugated |
| Immunogen | Purified IgM isolated from Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |