Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP
| Applications | Sandwich ELISA: 1/120,000 dilution of antibody was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate. Western blot (1/5000). Immunoelectrophoresis. |
| Reactivities | Chicken |
| Conjugation | HRP |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin
| Applications | ELISA. Western Blot (1/5000). Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate. |
| Reactivities | Chicken |
| Conjugation | Biotin |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP
| Applications | ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate. Western Blot (1/1000). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA. |
| Reactivities | Chicken |
| Conjugation | AP |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
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