C3 goat polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, IP, WB |
| Reactivities | Rat |
| Conjugation | Biotin |
| Immunogen | C3c isolated and purified from pooled normal Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgG2b (subclass specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2b antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2b isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2b in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000. |
| Reactivities | Rat |
| Conjugation | Biotin |
| Immunogen | Pools of purified homogenous IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Fibrinogen goat polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, IP, WB |
| Reactivities | Rat |
| Conjugation | Biotin |
| Immunogen | Fibrinogen is isolated from fresh plasma after removing Prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgE (Fc specific) goat polyclonal antibody, Biotin
| Applications | This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemistry and Cytochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Rat |
| Conjugation | Biotin |
| Immunogen | Purified homogenous IgE isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Alb goat polyclonal antibody, Biotin
| Applications | ELISA, IF, IHC, IP, R, WB |
| Reactivities | Rat |
| Conjugation | Biotin |
| Immunogen | Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from rat serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
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