Antibodies

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Monkey IgG (Fc specific) goat polyclonal antibody, FITC

Applications Can be used:
• In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates.
• To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilution: 1/20-1/80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled Rhesus Monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgG (H+L chain) goat polyclonal antibody, FITC, Lyophilized

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluoresence: 1/1,000-1/5,000.
Fluorescent Western Blot.
Reactivities Rabbit
Conjugation FITC
Immunogen Rabbit IgG, whole molecule.

Mouse IgG (Fc specific) goat polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunofluorescence: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG F(c) fragment.

Mouse IgA (alpha chain specific) goat polyclonal antibody, FITC

Applications FLISA: 1/100–1/400.
Flow Cytometry: ≤ 1 μg/106 cells.
Immunofluorescence: ≤ 1 μg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.

Mouse IgG1 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry: neat-1/10.
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG1 paraproteins.

Guinea Pig IgM (Fc specific) goat polyclonal antibody, FITC

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/10 - 1/40.
Reactivities Guinea Pig
Conjugation FITC
Immunogen Purified IgM isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgE (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in Human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80, depending on the method used.
Reactivities Human
Conjugation FITC
Immunogen Pools of purified homogenous IgE isolated from Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG1 Antibody Fluorescein Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:200 - 1:500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG heavy chain

Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:200 - 1:500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG2a heavy chain

Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed

Applications WB: User Optimized
IHC: User Optimized
IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG whole molecule

Human IgA (Secretory component) goat polyclonal antibody, FITC

Applications Can be used as reagent for the direct detection of secretory component in human cells, tissues and body fluids in Immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Reactivities Human
Conjugation FITC
Immunogen Purified free Human Secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Rabbit IgA (Fc specific) goat polyclonal antibody, FITC

Applications In immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation FITC
Immunogen Pools of purified IgA isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG3 (subclass specific) goat polyclonal antibody, FITC

Applications This antibody can be used to identify and measure IgG3, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Reactivities Mouse
Conjugation FITC
Immunogen Pools of purified homogenous IgG3 isolated from pooled Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/120.
Reactivities Monkey
Conjugation FITC
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80, depending on the method used.
Reactivities Monkey
Conjugation FITC
Immunogen Pools of purified IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.