Mouse Kappa Light Chain (free and bound) goat polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, WB |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
C3 goat polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, IP, WB |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | C3c isolated and purified from pooled normal Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Fibrinogen goat polyclonal antibody, Biotin
| Applications | ELISA, ID, IF, IHC, IP, WB |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Fibrinogen is isolated from fresh plasma after removing Prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG3 (subclass specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1.000 - 1/5,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Pools of purified homogenous IgG3 isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgE (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Purified homogenous IgE isolated from Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: For histochemical and Cytochemical use are usually between 1/100 and 1/250 In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Purified homogenous IgM isolated from Mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure. |
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