Secondary Antibodies

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Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482HR-N

USD 705.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
depending on the method used.
Reactivities Mouse
Conjugation HRP
Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612P

USD 300.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western blot: 1/2,000-1/10,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277BT-N

USD 380.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5,000-1/20,000.
Immunoblotting. 
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation Biotin
Mouse IgG2a (heavy chain) Rat Monoclonal Antibody [Clone ID: LO-MG2a-7]
SM1018P

USD 575.00

5 Days

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Purified

Applications ELISA: 5µg/ml as coating antibody.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG (H+L chain), adsorbed Goat Polyclonal Antibody
SP1041F

USD 910.00

5 Days

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50µl of 1/50-1/200 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC
Mouse IgG (H+L chain), Fab Fragment Goat Polyclonal Antibody
R1389P

USD 330.00

3 Days

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
For use in ELISA a dilution of 1:3,000-1:12,000 is recommended.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP32278RP-S

USD 355.00

3 Days

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE
Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612F

USD 330.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA: 1/10,000-1/50,000.
Western blot: 1/1,000-1/5,000.
Immunofluorescence: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation FITC
Mouse IgG1 (heavy chain) Rat Monoclonal Antibody [Clone ID: LO-MG1-2]
SM1017F

USD 820.00

5 Days

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, FITC

Applications Flow Cytometry: Use 10 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC
Human IgG1 Rabbit Polyclonal Antibody
AP20578PU-N

USD 445.00

3 Weeks

Human IgG1 rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Mouse IgD (Fc specific) Goat Polyclonal Antibody
AP21481AF-N

USD 705.00

2 Weeks

Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277FC-N

USD 380.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, FITC

Applications FLISA: 1/100–1/400.
Flow Cytometry: ≤ 1 μg/106 cells.
Immunofluorescence: ≤ 1 μg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC
Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612HRP

USD 400.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, HRP

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000
Western blot: 1/1,000-1/5,000
Flow Cytometry: 1/500-1/2,500
Reactivities Mouse
Conjugation HRP
Mouse IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed
TA397864S

USD 295.00

5 Days

Mouse IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 425
Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485AF-N

USD 705.00

2 Weeks

Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Unconjugated