Secondary Antibodies

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Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

mIgG (1,2a,2b,3) Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9F11

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse, Rat
Conjugation Unconjugated

mIgG1 Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR8A7

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG2a Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR6C3

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG3 Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR8B8

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG2a and mIgG2b Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9H1

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG2a Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9D3

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG2b Fcγ Fragment Specific Rabbit monoclonal antibody,OTIR13C2

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western blot: 1/2,000-1/10,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Purified

Applications ELISA: 5µg/ml as coating antibody.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Aff - Purified

Applications Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
For use in ELISA a dilution of 1:3,000-1:12,000 is recommended.
Reactivities Mouse
Conjugation Unconjugated

Human IgG1 rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human, Mouse, Rat
Conjugation Unconjugated

Mouse IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase Immunochemistry. When applied in any Cytochemical or Histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Unconjugated